DNA Release Dynamics from Bioreducible Layer-by-Layer Films
نویسندگان
چکیده
منابع مشابه
DNA release dynamics from bioreducible layer-by-layer films.
DNA release dynamics from layer-by-layer (LbL) films is an important aspect to consider with regards to localized gene delivery systems. The rate of DNA release and the condensation state of DNA during release are of particular interest in the field of gene delivery. A hyperbranched poly(amido amine) (RHB) containing bioreducible disulfide bonds is used to form interpolyelectrolyte complexes wi...
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Layer-by-layer (LbL) films containing cationic polyelectrolytes and anionic bioactive molecules such as DNA are promising biomaterials for controlled and localized gene delivery for a number of biomedical applications including cancer DNA vaccine delivery. Bioreducible LbL films made of disulfide-containing poly(amido amine)s (PAAs) and plasmid DNA can be degraded by redox-active membrane prote...
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. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Autobiographical Statement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
متن کاملCross-linked bioreducible layer-by-layer films for increased cell adhesion and transgene expression.
The effect of cross-linking layer-by-layer (LbL) films consisting of bioreducible poly(2-dimethylaminoethyl methacrylate) (rPDMAEMA) and DNA is examined with regard to rigidity, biodegradability, cell adhesion, and transfection activity using 1,5-diiodopentane (DIP) cross-linker. DIP chemically reacts with the tertiary amines of rPDMAEMA, altering the chemical composition of these LbL films. Th...
متن کاملDNA release dynamics from bioreducible poly(amido amine) polyplexes.
The DNA release dynamics of bioreducible poly(amido amine) polyplexes were studied in real time by atomic force microscopy (AFM). DNA release is triggered by a depolymerization of high-molecular-weight polycations into low-molecular-weight oligocations that occurs by means of the thiol and disulfide exchange reaction mechanism. AFM images were captured in a simulated physiological reducing envi...
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ژورنال
عنوان ژورنال: Langmuir
سال: 2010
ISSN: 0743-7463,1520-5827
DOI: 10.1021/la904673r